Journal: iScience

Article Title: Human iPSC cardiomyocyte patch transplantation modifies extracellular matrix and fibroblast behavior after myocardial infarction

doi: 10.1016/j.isci.2026.115341

Figure Lengend Snippet: TGF-β secreted from the hiPSC-derived CM patch induced Col1a1 expression but not Col3a1 (A) Schematic representation of the co-culture system. hiPSC-derived CMs were seeded on the upper chamber, whereas the cardiac fibroblasts isolated from mice heart were cultured at the lower chamber in the presence and absence of SB431542, a TGF-β receptor inhibitor. (B) RT-qPCR analysis of Col1a1 , Col3a1 , and Pai1 in the cultured cardiac fibroblasts. Two-way ANOVA (co-culture × inhibitor) with interaction; Tukey-adjusted post hoc tests on estimated marginal means. Data represent mean ± SEM. N = 3–6, one-way ANOVA followed by Tukey’s HSD test, p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.

Article Snippet: Then proteins were analyzed using primary antibodies for Col1a1 (R1038, OriGene, Rockville, MD), Col3a1(ab6310, Abcam, Cambridge, UK) and Gapdh (AM4300, Thermo Fisher Scienctific, Waltham, MA), respectively on Amersham Imager 600 (GE Healthcare, Chicago, IL).

Techniques: Derivative Assay, Expressing, Co-Culture Assay, Isolation, Cell Culture, Quantitative RT-PCR